Coliban Water has completed repairs to sewers in Bendigo after two blockages caused overflows in Jackass Flat and Long Gully.
Tree roots in the sewers have been identified as the cause of the two blockages and subsequent overflows.
The overflow in Jackass Gully, occurred in a 400 metre pipe, along Jobs Gully Road.
Due to the size of the sewer main, the volume of the overflow is estimated to be approximately 300 kilolitres.
Warning signs are in place at the site and the sewer will be monitored by Coliban Water in the future.
The second overflow occurred from a manhole on a 225mm sewer main into a 300m length of Long Gully Creek. It is estimated around 20kl of overflow occurred.
The EPA was notified about both incidents and Coliban Water will carry out CCTV investigations on the sewer mains.